Epinegetics, methylation, biologic age testing

[Time2]

Has anyone ever done an age test measuring methylation?
I compiled a short list of companies that I think do the methylation test, looking for others. Any discusion welcome.





https://epiagingusa.com/
https://epigenexperts.ca/product/bio...e-test-epiage/ (Canada)
https://yuniquemedical.com/anti-agin...al-age-truage/ ( I can’t tell what they measure, no mention of methylation)
https://muhdo.com/the-science/epigenetics/ (Europe?)
https://trudiagnostic.com/products/t...en-greenfield/

[sengsational]

When I saw the thread title, I said to myself "That's got to be Time2"


I don't have as much to share as you probably already know, but if there's discussion on it, I'd like to hear it and learn more.

[Time2]

Ya, I'd like to have my age measured using what is known as DNA Methylation, (Horvath clock) so I can see if the regimen I'm on has any helpful affects.

2015 paper by Horvath.
https://pubmed.ncbi.nlm.nih.gov/24138928/
I hear Dr. Sinclair say it will measure humans, which the 2015 paper only went as far as chimpanzes.
The above pubmed paper above does list many other articles on DNA Methylation.
Please post anything you glean from them.

[rodi]

Hmmm. I don't know a ton about methylation - other than it has something to do with how your body gets rid of toxins, handles homocystienes, and breaks down stuff to make the neurotransmitters happy.

The reason I know that last bit is that my family has MTHFR gene mutations so we can't methylize stuff as efficiently. I have one mutation, hubby has the other mutation, one son has the same mutation as me, and the other son has both mutations. This means we don't process folate or folic acide (b9) into methyl folate as efficiently ... Which is what crosses the brain barrier and keeps your neurotransmitters happy. It also means we don't efficiently convert b12 into methyl colbane... which is also needed for happy brains. We solve this by taking pre-methylized versions of b9 and b12.

Life is much better for the whole family once we confirmed these gene mutations and started taking bio-available vitamins.

[rodi]

Adding... The MTHFR gene mutation means your body can't produce the correct methylenetetrahydrofolate amino acid - which is needed for the methylization process.

[6miths]

Quote:

Originally Posted by Time2 Ya, I'd like to have my age measured using what is known as DNA Methylation, (Horvath clock) so I can see if the regimen I'm on has any helpful affects.

How would you know? Helpful or harmful?

[Time2]

Quote:

Originally Posted by 6miths How would you know? Helpful or harmful?

If measuring methylation is a good measure of your biological age, then trying an age reducing regimen, then retesting to see if it reduces your biological age.


I'm still trying to understand it, but "I think"
you lose methylation as you age, the less methylation you have the more biologic processes that can go haywire. (aging)

Here's the simplest explanation I have found, (2015)

https://www.mindbodygreen.com/0-1824...tion-more.html

This article has additional info, (2018)



https://www.healthline.com/health/methylation

[David]

Yes, there are few companies offering this test..
Actual Steve Horvath clock is done by Zymo Research mydnage.com
Another companies:
epi-age.com
epiagingusa.com
epigenexperts.ca
elysiumhealth.com/en-us/
muhdo.com
chronomics.com not sure if they are still doing.

All these companies do methylation test and measure epigenetic age
What is the difference:
1. The source of the biological material: blood, urine, saliva
2. The prices varies from 145 to 600 and more
3. Target genes that are used to measure epigenetic age.

hope it helps.

Quote:

Originally Posted by Time2 Has anyone ever done an age test measuring methylation? I compiled a short list of companies that I think do the methylation test, looking for others. Any discusion welcome. https://epiagingusa.com/ https://epigenexperts.ca/product/bio...e-test-epiage/ (Canada) https://yuniquemedical.com/anti-agin...al-age-truage/ ( I can’t tell what they measure, no mention of methylation) https://muhdo.com/the-science/epigenetics/ (Europe?) https://trudiagnostic.com/products/t...en-greenfield/

[David]

Not exactly. The Methylome (DNA methylation profile of the cell) changes with the age-yes. Some genes get methylated with a time, some genes lose methylation.
Is it possible to reverse aging?
One study showed that it is possible:
Gregory M. Fahy et al. Agig Cell. “Reversal of epigenetic aging and immunosenescent trends in humans"
But it is a case study- nine volunteers and requires deeper investigation.

Another paper showing that the diet and lifestyle can reverse aging by 3.25 years
"Reversal of Epigenetic Age with Diet and Lifestyle in a Pilot Randomized Clinical Trial"

These are actually only studies known demonstration age reversal with intervention.
Honestly, it is very premature to discuss the impact of any type of intervention on biological age.

Quote:

Originally Posted by Time2 If measuring methylation is a good measure of your biological age, then trying an age reducing regimen, then retesting to see if it reduces your biological age. I'm still trying to understand it, but "I think" you lose methylation as you age, the less methylation you have the more biologic processes that can go haywire. (aging) Here's the simplest explanation I have found, (2015) https://www.mindbodygreen.com/0-1824...tion-more.html This article has additional info, (2018) https://www.healthline.com/health/methylation

[Boho]

Quote:

Originally Posted by rodi ...We solve this by taking pre-methylized versions of b9 and b12. Life is much better for the whole family once we confirmed these gene mutations and started taking bio-available vitamins.

I have MTHFR "mutations" too. I searched for the word "methylation" in the data I got from entering my 23andMe raw data into Promethaese. I have 65% efficiency in processing folic acid. My mutation is found in approximately:

• 48% of Hispanic Americans
• 45% of Caucasian Americans
• 45% of Japanese
• 37% of Germans
• 29% of Asians
• 24% of African Americans
• 12% of Sub-Saharan Africans

It's so common it makes me wonder whether it has a benefit, but Promethaese says it has "bad repute" and:

Quote:

Our Take on The MTHFR Gene," is a 23andMe blog posting (January 5, 2017), a meta-analysis finding that the past two decades of scientific evidence as it relates to specific MTHFR-influenced health conditions to be inconclusive or conflicting, with two exceptions, 1. women with two copies of C677T variant, 2. a very rare variant that may cause homocystinuria. Their takeaway, "Based on the existing data, scientists at 23andMe have concluded that people should not interpret their genotypes at the common MTHFR variants as having an effect on their health." This reduced activity (i.e. this SNP) has been linked [13] disorders (though not necessarily reproducibly)

And my pet peeve of the day is poor writing. The first study I read on this was this open access article. The results and conclusions sections say folate and vitamin B12 supplementation had an effect, but they didn't say good or bad! Now I have to dig into this more and decide whether to take a supplement.